Research tool
Reconstitution Calculator
Work out the concentration after mixing and the units to draw for any dose. Reference figures for laboratory handling only.
Your vial
The syringe scales the draw-reference table below.
Result
Draw reference
Units and volume to draw for a range of common doses at this concentration. Doses past your syringe’s capacity are marked —.
| Dose | Draw (units) | Volume |
|---|
How much water to add
Standard reconstitution volumes by vial size. Tap a row to load it into the calculator above.
| Vial | Water | Concentration | 1 unit | Best for |
|---|---|---|---|---|
| 2 mg | 1 mL | 2,000 mcg/mL | 20 mcg | Semax, Selank, DSIP & small-dose peptides |
| 5 mg | 1 mL | 5,000 mcg/mL | 50 mcg | Higher-dose protocols |
| 5 mg | 2 mL | 2,500 mcg/mL | 25 mcg | Easier measurement for smaller doses |
| 10 mg | 2 mL | 5,000 mcg/mL | 50 mcg | BPC-157, TB-500, CJC-1295 — standard |
| 15 mg | 3 mL | 5,000 mcg/mL | 50 mcg | Thymosin Alpha-1, Epithalon, larger vials |
| 20 mg | 2 mL | 10,000 mcg/mL | 100 mcg | Retatrutide — 1 unit = 0.1 mg |
| 30 mg | 3 mL | 10,000 mcg/mL | 100 mcg | Retatrutide large vial |
| 50 mg | 5 mL | 10,000 mcg/mL | 100 mcg | High-volume research vials |
Mixing, step by step
- Wipe the bacteriostatic-water vial septum with an alcohol swab; let it dry ~10 seconds.
- Draw 2 mL of bacteriostatic water into a sterile syringe.
- Wipe the peptide vial septum with a fresh swab and let it dry.
- Insert the needle at a 45° angle and inject the water slowly down the inside wall — don’t aim the stream at the powder.
- Gently swirl or roll the vial between your palms until fully dissolved. Don’t shake or vortex.
- The solution should be clear and colourless. If it’s cloudy or has particles, don’t use it.
- Label the vial with today’s date and the concentration: 5,000 mcg/mL.
- Refrigerate at 2–8°C (don’t freeze). Use within 4–6 weeks.
The formula
Each insulin unit on a U-100 syringe is 0.01 mL, so units = mL × 100. A 0.5 mL (U-50) or 0.3 mL (U-30) barrel just caps the maximum draw at 50 or 30 units.
Frequently asked questions
What is bacteriostatic water and why is it required?
How much BAC water should I add?
What is a U-100 insulin syringe?
How long does a reconstituted peptide remain stable?
Reconstitution Calculator
Welcome to the Pepnerd Reconstitution Calculator, your essential tool for accurately preparing your research peptides. Getting the dosage right is paramount in peptide research, and incorrect reconstitution is one of the most common pitfalls for beginners. This calculator simplifies the complex maths involved, ensuring you can confidently and precisely measure your doses every time.
Understanding the correct dilution ratio is not just about accuracy; it's about the integrity of your research. A miscalculated dose can lead to unreliable results, wasted product, and potentially compromise your entire study. Our calculator is designed to eliminate guesswork, providing clear, actionable instructions so you can focus on your research with peace of mind. For a comprehensive overview, refer to our full reconstitution guide.
How to Use the Calculator
Our Reconstitution Calculator is straightforward to use, requiring just three key pieces of information to provide you with an accurate dosage measurement. You will need to input the following:
- Vial Size (mg): The total amount of peptide contained in your vial, typically measured in milligrams.
- Bacteriostatic Water Volume (mL): The exact volume of bacteriostatic water you plan to add to your peptide vial, measured in millilitres.
- Desired Dose (mcg): The specific amount of peptide you wish to administer per dose, measured in micrograms.
Once these values are entered, the calculator will output the number of units on a U-100 insulin syringe required to achieve your desired dose. This ensures precise measurement, especially crucial for potent peptides.
Worked Examples
To illustrate how the calculator works, let's walk through a couple of common scenarios:
Example 1
Imagine you have a 5mg vial of peptide, and you add 2mL of bacteriostatic water. If your desired dose is 250mcg, the calculator will show that you need to draw up to the 10 units mark on a U-100 insulin syringe.
Example 2
Consider a 10mg vial of peptide, reconstituted with 2mL of bacteriostatic water. If you require a 500mcg dose, the calculator will again indicate that you need to draw up to the 10 units mark on a U-100 insulin syringe.
The Reconstitution Formula
For those who prefer to understand the underlying maths, the formula used by our calculator is as follows:
Units = (Desired dose mcg ÷ Total mcg in vial) × mL of water added × 100
This formula accounts for the concentration of your reconstituted peptide and translates it into measurable units on a standard U-100 insulin syringe. For more detailed information on peptide terminology, visit our Peptide Glossary.
About Bacteriostatic Water
Bacteriostatic water is crucial for proper peptide reconstitution, as it helps to preserve the peptide and prevent bacterial growth, extending its shelf life. Always use high-quality bacteriostatic water for your research. Learn more about its importance and the full reconstitution process in our comprehensive Reconstitution Guide.
Further Resources
Ensuring accurate dosing and safe handling practices are key to successful peptide research. Explore our resources for more information: