NAD+ (Injectable)

Half-life

~45 minutes

Very short

minsdays

Route

SubQ injection

Evidence

Early human data

Risk

Standard

Overview

Known for

Nicotinamide Adenine Dinucleotide. Essential coenzyme for cellular energy production, DNA repair, and sirtuin activation. Declines significantly with age. Injectable NAD+ bypasses digestive degradation. Improves energy, mental clarity, and may slow aging.

Mechanism

Coenzyme in every cell — critical for energy metabolism, DNA repair, sirtuin activation. Declines ~50% from age 20 to 50. IV bypasses poor oral bioavailability.

Key facts

Format

Lyophilized powder

Mechanism targets

Classification

Secondary

Supply & nature

Natural / Topping UpNatural

FDA category

Not reviewed (supplement)

Evidence & standing

Evidence base

Early human data

Risk level

Standard

Best studies

6 references

Yoshino et al. (2018) Cell Metab 27:529-47 — NAD+ metabolism review
Rajman et al. (2018) Cell Metab 27:529-47 — NAD+ and healthspan
Mills et al. (2016) Cell Metab 24:795-806 — NMN in aging mice
IV NAD+ lacks large-scale human RCTs
NOT FDA APPROVED as injectable therapeutic
Oral precursors (NR, NMN) more commonly studied in humans

Dosage & protocol

Typical dose

250-500mg

Research range

250–500 mg

Cycle

5-day loading / monthly maintenance

Onset

0 days first effects

Full effect

21 days

Half-life

~45min

~45 minutes (IV NAD+ cleared rapidly) | SubQ: variable

Protocol

IV: 250-500mg per session (clinical setting). SubQ/IM: 50-100mg daily or 2-3x per week.

Time to effects

EARLY: Immediately-24hrs

FULL: 2-4 weeks

IV infusion: Effects felt DURING the session — increased energy, mental clarity, sometimes euphoria. Also potential nausea, chest tightness (infuse slowly).

SubQ/IM: Within 24-48 hours — improved energy, mental clarity, reduced brain fog.

Week 1-2: Significantly improved energy levels. Better focus. Reduced fatigue.

Week 2-4: Cumulative cellular benefits. Improved exercise recovery. Enhanced cognitive function.

One of the fastest 'felt' peptides. Many report immediate effects from IV and within days from SubQ. Minimum commitment: 2-4 weeks for sustained benefits.

Recommended vial

500mg vials

At 100mg/day, 100mg = 1 day (impractical). 500mg = 5 days ✓. 1000mg = 10 days ✓. 500mg is practical minimum.

Reference figures only

Dosing reflects published laboratory protocols and is not guidance for use in any living subject.

Safety

Reversibility

Fully Reversible

Side effects

IV: chest tightness/pressure (30-40%, dose-rate dependent), nausea, flushing, headache, cramping. SubQ: injection site discomfort.

Interactions

Alcohol (depletes NAD+), chemotherapy drugs (PARP inhibitors interact — olaparib etc.), medications metabolized by NAD+-dependent enzymes

Handle with research caution

No strict contraindications with other peptides on this list.

Storage & handling

Lyophilised

12–24 months

room temp

Reconstituted

4–6 weeks

2–8°C

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